Serotype Variations and Distributions of Dengue Virus During the 2022 Outbreak in Chattogram, Bangladesh
Abstarct:
Introduction: In the year 2022, a Dengue virus outbreak occurred nationwide in Bangladesh, including Chattogram, a port city. The purpose of this study was to identify and document the serotypes of Dengue virus and to measure the viral load of the virus positive specimens.
Methods: This observational study was conducted in a tertiary care hospital of Chattogram during a period of six months from June through December, 2022. A total of 74 patients with symptoms suggestive of Dengue Fever(DF) and NS1 or IgM/IgG positive dengue patients were recruited into the study. From them clinicopathological data was collected on a case record form(CRF). Ten ml of venous whole blood was drawn in EDTA tubes then centrifuged and was sent to the laboratory at SlieaGen L.L.C in Austin, Texas, USA, for Dengue serotyping and viral load analysis by qPCR-LDT method. A total of 73 specimens were analyzed, one specimen was excluded. Ethical clearance was obtained from the hospital ethical review board, written informed consents were also taken from all subjects. Serotyping and qPCR testing data was shared by SlieaGen LLC once analysis was completed.